SHROOM2 antibody (AA 1295-1344)

Catalog No. ABIN6740410

The Rabbit Polyclonal anti-SHROOM2 antibody has been validated for WB. It is suitable to detect SHROOM2 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Chicken, Monkey and Pig.

Quick Overview for SHROOM2 antibody (AA 1295-1344) (ABIN6740410)

Target: SHROOM2 (Shroom Family Member 2 (SHROOM2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHROOM2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SHROOM2 Antibody

Binding Specificity: AA 1295-1344

Specificity: Human SHROOM2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog (100%) Horse, Guinea pig, Chicken (92%) Bovine (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1295-1344 of human SHROOM2 (Q13796, NP_001640). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Bat, Horse (92%), Xenopus (90%), Platypus, Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SHROOM2

Target: SHROOM2 (Shroom Family Member 2 (SHROOM2))

Alternative Name: SHROOM2

Background: Name/Gene ID: SHROOM2

Synonyms: SHROOM2, Apical-like protein, HSAPXL, Protein APXL, Protein Shroom2, APX homolog of Xenopus, APXL, Shroom family member 2

Gene ID: 357

NCBI Accession: NP_001640

UniProt: Q13796

Pathways: Cell-Cell Junction Organization, Asymmetric Protein Localization